“Naturalization” of Routine Assisted Reproductive Technologies by In Vitro Culture of Embryos with Microvibration: Sex Ratio, Body Length, and Weight of 2,456 Live-Birth Deliveries after Transfer of 9,624 Embryos In Vitro Cultured in Static System and with Microvibration

Vladimir Isachenko,¹ Karl Sterzik,² Evgenia Isachenko,¹ Robert Maettner,² Plamen Todorov,³ Gohar Rahimi,¹ Peter Mallmann,¹ Erwin Strehler,² Igor Pereligin,⁴ Jos Luis Alabart,⁵ and Markus Merzenich⁶

1. Research Group for Reproductive Medicine and IVF-Laboratory, Department of Obstetrics and Gynecology, Cologne University, Kerpener Str. 34, 50931 Cologne, Germany
2. Department of Reproductive Medicine, Christian-Lauritzen Institute, Frauenstr. 51, 89073 Ulm, Germany
3. Institute of Biology and Immunology of Reproduction, Tzarigradsko Shosse 73, 1113 Sofia, Bulgaria
4. IVF Center “Genesis Dnepr”, 119-120 Rybinska St., Dnipro, Ukraine
5. Servicio de Investigaci on Agroalimentaria (DGA), Avda. Monta˜nana 930, 50059 Zaragoza, Spain
6. MedEvent Dr. Merzenich GmbH, Im Zollhafen 12, 50678 Cologne, Germany

Correspondence should be addressed to Vladimir Isachenko; This email address is being protected from spambots. You need JavaScript enabled to view it.

Received 22 September 2017; Revised 13 November 2017; Accepted 5 December 2017; Published 20 December 2017

Academic Editor: Brad Upham

Abstract

Aim was to determine whether there is any difference in the sex ratio, body length, and body weight of 2,456 deliveries after transfer of 9,624 embryos derived using in vitro culture under static and mechanical microvibration conditions. Pronuclear embryos from 4435 patients were cultured in vitro under two different conditions: without () and with mechanical agitation (). Sex ratio, body length, and weight of 2,456 live-birth deliveries after transfer of 9,624 embryos were noted. The proportion of males at birth was significantly associated with mode of in vitro culture of embryos only among women aged 40 years and older. The rate “body length” was significantly associated with mode of in vitro culture of embryos only among women aged 29 and younger. In the same time, among twins, this ratio positively associated with in vitro culture of embryos under microvibration only among women aged 30–34 years as well as ≥40 years and negatively among women aged 35–39 years. It was concluded that birth weight of infants was positively associated with mode of in vitro culture of embryos under microvibration among women of all age groups. This trial registration number is ISRCTN13773904, registered 6 April 2016.

Research Article | Open Access Volume 2017 |Article ID 4964053 | https://doi.org/10.1155/2017/4964053