Thursday, 17 November 2016 10:06
Published in IVF Research

In vitro Micro-Vibration Increases Implantation Rate after Embryonic Cell Transplantation

Vladimir Isachenko1; Karl Sterzik2; Robert Maettner2; Evgenia Isachenko1; Plamen Todorov3; Gohar Rahimi1; Peter Mallmann1; Erwin Strehler2; Igor Pereligin4; José Luis Alabart5; Markus Merzenich6

1 University Maternal Hospital, Department of Obstetrics and Gynecology, Cologne University, Cologne, Germany
2 Department of Reproductive Medicine, Christian-Lauritzen Institute, Frauenstr. 51, 89073 Ulm, Germany
3 Institute of Biology and Immunology of Reproduction, Sofia, Bulgaria
4 IVF Center “Genesis Dnepr”, Ukraine
5 Servicio de Investigación Agroalimentaria (DGA), Zaragoza, Spain
6 Praxisklinik, Schoenhauser Str. 3, 50968 Cologne, Germany
* Address correspondence to Vladimir Isachenko, Dr.(SU), University Maternal Hospital, Department of Obstetrics and Gynecology, Cologne University, Kerpener Str. 34, 50931 Cologne, Germany Tel: +49-221-4784924; Fax: +49-221-4785247; E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Copyright © 2016 Cognizant Communication Corporation

Abstract: In natural conditions the oocyte and embryo are subjected to ever changing dynamic processes. However, the routine assisted reproductive technologies today involve the use of static in vitro culture systems. Objective was to determine whether there is any difference in the viability of embryos after in vitro culture under static and mechanical micro-vibration condition. It was evaluated the viability of embryonic cells (9,624 embryos) generated from 4,436 couples after in vitro culture. For Groups <29 years, 30-34 years, 35-39 years and >40 years, the following rate of high quality embryos without fragmentation (2 to 4 blastomeres on Day 2; 6 to 8 blastomeres and compacting morula on Day 3; blastocyst, expanded and hatching blastocyst on Day 5) was detected (static vs. vibration, respectively): 65% vs. 71%, 44% vs. 69%, 67% vs. 76% (for statistic significant differences between respective rates in these three groups P<0.05) and 67% vs. 66% (P>0.1). The following baby-take-home rate was detected for Groups <29 years, 30-34 years, 35-39 years and >40 years, (static vs. vibration, respectively): 30% vs. 31% (P>0.1, increasing only on the level of tendency), 28% vs. 37%, 23% vs. 29% and 9% vs. 15% (differences between respective rates in these three groups with P<0.05). It was concluded that in vitro culture of embryos under micro-vibration (with a mimic of conditions in nature whereby oviductal fluid is mechanically agitated by the epithelial cilia) significantly increases baby-take-home rate for patients of 30 years and older.

Key words: Baby-takehome rate; Embryonic cells transplantion; In vitro culture; Micro-vibration

Source: Cell Transplantation

Publisher: Cognizant Communication Corporation

Cell Transplant. 2016 Oct 7. [Epub ahead of print]

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Last modified on Tuesday, 18 July 2017 10:30

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